
FANR 6750
Fall 2026
In the last lecture, we learned that statistics is what allows us to make inferences about a population in the face of uncertainty
Statistical inference requires models
An abstraction of reality used to describe the relationship between two or more variables
Many types (conceptual, graphical, mathematical)
In this class, we will deal with statistical models1
Mathematical representation of our hypothesis
Explicitly measure and account for uncertainty
By necessity, models will be simplifications of reality (“all models are wrong…”)
Do not have to be complex
Inference requires models
Models link observations to processes
Models are tools that allow us understand processes that we cannot directly observe based on quantities that we can observe
Suppose we are interested in the hypothesis that water availability limits acorn production by oak trees

\[\LARGE y = a + bx\]

It may not be obvious, but this is essentially the only model we will use this semester2
\[\LARGE y = a + bx\]
If we want to use this as a statistical model, what’s missing?
Stochasticity
\[\LARGE y = a + bx\]
If we want to use this as a statistical model, what’s missing?
Stochasticity
\[ response = deterministic\; part+stochastic\; part\]
\[\underbrace{\large E[y_i] = \beta_0 + \beta_1 \times x_i}_{Deterministic}\]
\[\underbrace{\large y_i \sim normal(E[y_i], \sigma)}_{Stochastic}\]
Sometimes you will see linear models written as:
\[\Large y_i = \beta_0 + \beta_1 x_i + \epsilon_i\]
\[\Large \epsilon_i \sim normal(0, \sigma)\]
with the \(\large \epsilon_i\) terms referred to as residuals or residual error
Mathematically, the two formulations are identical
We will use both formulations
Throughout the semester, we will try to be consistent with mathematical notation, e.g.
To the extent possible, these follow conventions used in many textbooks/papers (but there is a lot of variation between authors)
Remember that these are just symbols - you could replace them with other symbols (e.g., emoji) and it would not change their interpretation!
\(\large \beta_0\) = intercept
\(\large \beta_n\) = slope
Mathematically, the interpretation of the intercept and slope(s) is always the same
Ecologically, the interpretation will depend on:
We will discuss each of these scenarios in detail as the semester progresses
A “simple” example
\[\underbrace{\large E[y_i] = -2 + 0.5 \times x_i}_{Deterministic}\]
\[\underbrace{\large y_i \sim normal(E[y_i], \sigma=0.25)}_{Stochastic}\]

A more complex model
\[\large y_i = \beta_0 + \beta_1x_{i1} + \beta_2x_{i2} + ... + \beta_px_{ip} + \epsilon_i\]
\[\large y = 20 + 0.5x - 0.3x^2\]
One concept we will talk about a lot is residuals, i.e., \(\epsilon_i\)

One concept we will talk about a lot is residuals, i.e., \(\epsilon_i\)
EVERY model has assumptions
\[\Large y_i = \beta_0 + \beta_1 x_i + \epsilon_i\]
\[\Large \epsilon_i \sim normal(0, \sigma)\]
\[\large y_i = \beta_0 + \beta_1x_i + \epsilon_i\] \[\large \epsilon_i \sim normal(0, \sigma)\]
\[\large y_i = \color{#D47500}{\beta_0 + \beta_1x_i} + \epsilon_i\] \[\large \epsilon_i \sim normal(0, \sigma)\]
\[\large y_i = \color{#D47500}{\beta_0 + \beta_1x_i} + \epsilon_i\] \[\large \epsilon_i \sim \color{#3CB521}{normal}(0, \sigma)\]
\[\large y_i = \color{#D47500}{\beta_0 + \beta_1x_i} + \epsilon_i\] \[\large \epsilon_i \sim \color{#3CB521}{normal}(0, \color{#CD0200}\sigma)\]
Remember that the normality assumption applies to the residuals not the data.
In the data below, the histogram of the response variable y shows that the data is clearly not normally distributed

Remember that the normality assumption applies to the residuals not the data.
In the data below, the histogram of the response variable y shows that the data is clearly not normally distributed
But a histogram of the residuals (green lines) shows that they are normal (or at least close)
Very flexible
Link different “tests” (e.g., t-tests, ANOVA, ANCOVA, linear regression)
Can be used for different statistical goals
Linear models allow us to quantify relationships between variables
Generally, models are fit to samples
The intercept and slope values correspond to the observed response and predictor values
If we repeated our study, the sampled values would change and so would the intercept/slope values
We’re generally interested in the population, not the sample
Inference about populations requires quantifying how well our estimated values represent the true population values (which we can never know)
Next time: Principles of statistical inference
Reading: Feiberg chp. 1.6-1.8
Though graphical models will play an important role later in the semester
With some minor variations, mainly in \(x\)
You know what happens when you assume…
Note that these assumptions apply to the residuals, not the data!